燒傷感染對血清中補體C_3和IL-10水平的影響

摘要： 目的分析燒傷感染對血清中補體C_3和IL-10水平的影響,旨在根據(jù)其變化水平及時給予合適的免疫調(diào)節(jié)治療,預防感染的發(fā)生。方法分別采集患者燒傷后第3、7、14天的創(chuàng)面分泌物和外周血,對分泌物做細菌培養(yǎng),用酶聯(lián)免疫吸附測定法(Enzyme-linked immunosorbent assay,ELISA)檢測血清中的補體C_3和IL-10水平,病情嚴重疑有膿毒血癥者及時進行血培養(yǎng)。結果燒傷后創(chuàng)面感染率不斷上升,膿毒血癥發(fā)生率達16.4%,對補體C_3水平的影響無統(tǒng)計學意義(P>0.05),但燒傷后第14天膿毒血癥患者血清中IL-10水平是創(chuàng)面感染患者的2倍,相比有統(tǒng)計學意義(P<0.05)。結論補體C_3水平升高有利于提高機體抵抗力,但不能反映機體的感染情況:IL-10水平過度升高對感染加重有一定的預警作用,在臨床判斷過程中輔助監(jiān)測血清中IL-10水平有助于發(fā)現(xiàn)感染加重的趨勢,以便及時給予免疫調(diào)節(jié)治療。
Objective To analyze the influence of burn infection on complement C_3 and IL-10 level in blood serum with the purpose of providing timely and proper immunoregulation based on the level changes and preventing the occurrence of infection.Methods Collect the secretion and the peripheral blood on the wounds respectively at the third,seventh and fourteenth day postbum.Do bacterial culture to the secretion and use ELISA method to test the levels of complement C,and IL-10 level in blood serum.For serious cases that were suspected with septicopyemia,the blood culture was performed immediately. Results The postburn wound infection rate increased continuously and the incidence of septicopyemia also reached 16.4%. But the effects they exerted on the level of complement C3 had no statistical significance(P>0.05).However,at the fourteenth day postbum,the level of IL-10 in blood serum of patients with septicopyemia was two times over that of the patients with burn infection,and the comparison had significance in statistics(P<0.05).Conclusion The increase of complement C_3 level can improve the body immunity but can not reflect the infection situation of the body,while the excessive increase of IL-10 level signifies the aggravation of infection to some extent,and in clinic,the auxiliary detection of IL-10 level in blood serum can help to discover the tendency of infection aggravation and so that prompt immunoregulation can be given.