燒傷病房耐甲氧西林金黃色葡萄球菌mec耐藥基因及HVR基因分型研究

摘要： 目的了解我院燒傷病房耐甲氧西林金黃色葡萄球菌(MRSA)基因型及其耐藥性,為我院燒傷病房MRSA感染的防治及分子流行病學(xué)研究提供實(shí)驗(yàn)依據(jù)。方法采用K-B瓊脂擴(kuò)散法做抗菌藥物敏感實(shí)驗(yàn),用聚合酶鏈反應(yīng)(PCR)方法擴(kuò)增MRSA mec基因以及MRSA mec基因高變區(qū)(HVR),根據(jù)擴(kuò)增片段大小的差異進(jìn)行基因分型,分析基因型與耐藥性的關(guān)系。結(jié)果我院燒傷病房耐甲氧西林金黃色葡萄球菌攜帶mec耐藥基因,mec基因攜帶率為87.5%,HVR分型只有A、C兩種分型,分別為20.3%、78.8%。結(jié)論我科燒傷病房耐甲氧西林金黃色葡萄球菌HVR分型只有A、C兩種分型,以C型多見(jiàn)。
Objective To explore the genotype and drug tolerance of MRSA(Methicinlin - resistant Staphylococus Aureus) in a purpose to provide the experimental evidence for prevention and treatment of MRSA infection in burn ward as well as studies on molecular epidermics.Methods Perform the antimicrobial medication sensitive test with K - B agar diffusion method,augment gene MRSAmec and its Hypervariable Region(HVR) by Polymerase Chain Reaction(PRC). And then analyze the relation between genotype and drug tolerance based on the size differences among augmented gene sections. Results There was MRSA carrying drug resistant gene mec in the bum ward of our hospital,with the carrying rate of mec gene being 87.5%.There were only type A and type C of HVR counting up 20.3%and 78.8%respectively.Conclusion There were only type A and type C in MRSAmec HVR in the burn ward of our hospital,and type C was the prevalent type.